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ForumsCOA & Analytical TestingLC-MS/MS vs HPLC for peptide analysis — November 2025 Page 9

LC-MS/MS vs HPLC for peptide analysis — November 2025

Dr.SportsMedIN Mon, Jul 22, 2024 at 3:37 PM 51 replies 2,260 viewsPage 9 of 11
labquiet_amy
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Dec 22, 2024 at 9:25 PM#41

The figures, for anyone assembling their own picture. If you are comparing against somebody else’s result, check that you are comparing the same measurement taken the same way. Most of the apparent contradictions in these threads dissolve at that step.

4 2TrialNerd_Beth, HPLC_Greg, LibrarianMeg and 1 other
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Dr.CardioMD
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Dec 29, 2024 at 1:22 PM#42

Following on from TrialNerd_Beth — and this may be the naive question:

What did you change at the same time, and can you separate the two now?

Last edited: Dec 29, 2024 at 7:22 PM
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Admin
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Jan 5, 2025 at 5:15 AM#43

Moderator note: two posts asking for a source have been merged into one. Please search the thread before asking again. Tagging this one for the weekly digest.

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LarryQC_SD
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Jan 11, 2025 at 9:04 PM#44
labquiet_amy said:
If you are comparing against somebody else’s result, check that you are comparing the same measurement taken the same way.

That holds where the measurement is reliable. Where it is noisy — and a lot of what gets tracked here is noisy — the same reasoning produces confident nonsense.

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Dr.MetabolicMD
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Jan 18, 2025 at 12:49 PM#45
labquiet_amy said:
If you are comparing against somebody else’s result, check that you are comparing the same measurement taken the same way.

That reframing is the part I needed.

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