LC-MS/MS vs HPLC for peptide analysis — November 2025

Found this through search years later and it is still the best discussion of the question, so adding one thing. For anyone reading later: the numbers in this thread are worth checking against a primary source before you act on them, including mine. Half the figures circulating in this community trace back to a secondary summary that dropped a qualifier.

bri_stats said:

One habit that pays for itself: post the method alongside the number.

Agreed, and the size of the effect matters as much as its existence. Something real and small gets treated here as though it were real and decisive.

bri_stats said:

One habit that pays for itself: post the method alongside the number.

Saving this. It is the first explanation that did not require me to already understand it. Printing the relevant bit and taking it with me.

Clinical perspective, offered as context rather than as advice. The mechanism and the magnitude are separate questions. Agreeing that something happens says nothing about whether it happens enough to act on.

Dr.ReproEndo said:

The mechanism and the magnitude are separate questions.

Second this.