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ForumsCOA & Analytical TestingHas anyone dealt with moisture content analysis for lyophilized peptides? Page 5

Has anyone dealt with moisture content analysis for lyophilized peptides?

AttorneyGrant Sat, Mar 14, 2026 at 11:18 PM 21 replies 910 viewsPage 5 of 5
RetaRick_CA
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Mar 21, 2026 at 1:26 AM#21

A narrower follow-up, since the general answer is now clear:

Did your prescriber agree with that reading, and if not what was their objection?

37 10SkepticalSean, Dr.CardioMD, EndoResFellow and 34 others
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sophie_paris
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Mar 22, 2026 at 1:20 AM#22
rachel_ABQ said:
Third-party testing services for certificates of analysis besides Janoshik: Janoshik: Gold standard for peptide community, ~$136, 10-14 day turnaround…

My HPLC chromatogram analysis for certificates of analysis — I sent my batch to Janoshik and here's what the results showed:

Main peak retention time: 15.6 minutes (consistent with semaglutide reference standard)
Peak purity: 99.4%
Related substances: 0.8% (single impurity at 9.3 minutes — likely a synthesis intermediate)
Mass spec: m/z 4113.6 (matches expected molecular weight)

This is a clean result. The mass spec confirmation is particularly important — it proves the molecule is actually semaglutide and not a substitution.

38 11CryptoCarl, MariaRD, AussieAnna and 35 others
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sarah.morrison
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Mar 23, 2026 at 1:13 AM#23
rachel_ABQ said:
Third-party testing services for certificates of analysis besides Janoshik: Janoshik: Gold standard for peptide community, ~$136, 10-14 day turnaround…

That is right, and it stops being right at the edges. The general case is well behaved; the interesting cases in this thread are all at the boundary where the general case breaks.

Last edited: Mar 23, 2026 at 2:13 AM
39 12tony_orlando, Dr.NephBHM_UK, kim_atl_prep and 36 others
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pam_stl
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Mar 24, 2026 at 1:06 AM#24
rachel_ABQ said:
Third-party testing services for certificates of analysis besides Janoshik: Janoshik: Gold standard for peptide community, ~$136, 10-14 day turnaround…

That reframing is the part I needed. I will report back once I have actually tried it.

Last edited: Mar 24, 2026 at 6:06 AM
40 13SleepDoc_PDX, RegAffairsDC, BiostatsBrad and 37 others
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anders_CPH
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Mar 25, 2026 at 12:58 AM#25

Clinical perspective, offered as context rather than as advice. Take it one variable at a time. Almost every unanswerable question in these threads is unanswerable because three things changed in the same fortnight, and no amount of subsequent argument can untangle them after the fact.

41 14ChrisMacros, KetoKyle, CanadaChris and 38 others
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