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ForumsDosing & ProtocolsMath is hard - help me with reconstitution please — May 2025 Page 2

Math is hard - help me with reconstitution please — May 2025

TrialTracker_MD Thu, May 16, 2024 at 7:05 AM 10 replies 2,033 viewsPage 2 of 2
MikeFit_NJ
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May 17, 2024 at 3:05 AM#6
sarah.morrison said:
Concentration choice is a precision decision, not a preference.

I am going to disagree with reconstituting low as a general rule. More diluent, more punctures, more in-use days at room temperature, and the stability trade-off is real. Precision is not the only variable being optimised.

2 22LeilaHI, marcus_mpls
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DataDave
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May 17, 2024 at 11:03 AM#7
TrialTracker_MD said:
I reconstituted a 10mg vial with 2ml of bacteriostatic water and I want someone to check my unit arithmetic before I draw anything, because I keep…

Peptide calculator app recommendation for dosing arithmetic: I use PeptideCalc to calculate reconstitution volumes and injection doses. Eliminates math errors completely.

Input: vial size (mg), desired concentration (mg/mL), prescribed dose (mg)
Output: BAC water volume (mL), injection volume (units on insulin syringe)

A math error with injectable medication can mean underdosing or overdosing. Use a calculator every time until the math becomes second nature.

1 21mike_nyc
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claudia_zurich
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May 17, 2024 at 7:01 PM#8
MikeFit_NJ said:
I am going to disagree with reconstituting low as a general rule.

Reconstitution tip for anyone mixing dosing arithmetic from lyophilized powder: use bacteriostatic water, NOT sterile water. The benzyl alcohol in BAC water preserves multi-dose vials for up to 28 days.

Inject the water slowly down the side of the vial. NEVER shake — gentle swirling only until fully dissolved. Should be crystal clear with no particles.

50 20Dr.LipidDallas, alex_tucson, kevin_tulsa and 47 others
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mia_MS2
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May 18, 2024 at 2:59 AM#9

One thing that is still open after ben_calgary’s answer:

How much material I am losing to dead space, and whether that explains why a 10mg vial gives me nine usable draws rather than ten?

49 19steve_okc, dave_SLC, FDA_TrackerJim and 46 others
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TrialTracker_MD
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May 19, 2024 at 5:14 PM#10

OP back with an update, since a thread like this is useless without one.

Resolved. I wrote mg, ml and mg/ml on the vial in marker and did the division in that order, and the two of us now get the same number every time.

35 8lori_vegas, Dr.PulmRoch, maya_sedona and 32 others
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