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ForumsCOA & Analytical TestingAmino acid analysis for peptide identity — looking for input

Amino acid analysis for peptide identity — looking for input

tom_AK Thu, May 16, 2024 at 5:30 PM 15 replies 2,058 viewsPage 1 of 3
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tom_AK
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Jan 2026
Anchorage, AK
May 16, 2024 at 5:30 PM#1

My appetite is suppressed enough that hitting a protein target is now the hardest part of the day, and everything I read assumes I can simply eat more.

The arithmetic that makes it tractable: reference weight in kg times 1.6 gives the target; divide by four for a per-sitting figure; then find four foods that hit that figure in a volume you can actually finish. Most people need two of the four to be liquid or semi-liquid early on.

What I am trying to establish is whether the RDA is the wrong reference entirely during rapid weight loss, and what the actual target should be.

I have searched first, so if this is covered somewhere point me at it and I will read it.

26 21steve_okc, dave_SLC, FDA_TrackerJim and 23 others
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Dr.NutriCornell
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May 16, 2024 at 5:35 PM#2

This one has a reasonably settled answer, so here it is. Distribution matters less than total but it is not nothing. Roughly 25 to 40g per sitting with enough leucine to trigger synthesis, three or four times a day, is more effective than the same total in one enormous evening meal — and on a suppressed appetite the enormous evening meal is the one you will not finish anyway.

25 20Dr.SleepRoch, laura_annarbor, JenMemphis and 22 others
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Dr.NephBHM_UK
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May 16, 2024 at 5:40 PM#3
Dr.NutriCornell said:
Distribution matters less than total but it is not nothing.

True, and anyone with reduced kidney function needs that target set by a clinician rather than a forum. High protein is safe in normal kidneys and is not automatically safe in impaired ones.

24 19PharmacoVig_BOS, SurmountFan_IN, PeptideChemSF and 21 others
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marco_milano
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May 16, 2024 at 5:45 PM#4
tom_AK said:
My appetite is suppressed enough that hitting a protein target is now the hardest part of the day, and everything I read assumes I can simply eat…

This matches mine closely enough to be worth saying so. On a suppressed appetite the winning strategy is protein density per unit of volume, not per calorie. Isolate powders, Greek yoghurt and cottage cheese, egg whites, lean fish, and tinned tuna all deliver a lot of protein in a small physical volume. Front-load it: appetite is usually least suppressed in the first hours after waking and worst on the day or two after dosing, so get the majority in early in the day and early in the week.

23 18tammy_FL, Dr.LipidDallas, alex_tucson and 20 others
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DebRD_ATL
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Atlanta, GA
May 16, 2024 at 6:12 PM#5

From the other side of the consultation, briefly.

DEXA scan update relevant to protein intake:

Scanned at months 0, 6, and 12. Here are the body comp numbers:

BaselineMonth 6Month 12
Total mass262 lbs232 lbs219 lbs
Fat mass122 lbs82 lbs54 lbs
Lean mass160 lbs153 lbs156 lbs
Body fat %39%33%23%

Lean mass preserved — actually GAINED 1-2 lbs thanks to progressive overload + 1.4g/kg protein. The protein intake discussion needs to include body comp data, not just scale weight.

22 17HealthEcon_DC, PedsEndoPhilly, SleepDoc_PDX and 19 others
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