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ForumsCOA & Analytical TestingAmino acid analysis for peptide identity — looking for input

Amino acid analysis for peptide identity — looking for input

rick_sfbay Tue, Mar 10, 2026 at 7:06 AM 14 replies 787 viewsPage 1 of 3
rick_sfbay
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Mar 10, 2026 at 7:06 AM#1

I tracked honestly for two weeks and averaged 68g of protein against a target of about 120g. That gap is the whole problem and no meal plan I have found is written for it.

What I actually want to know is whether the RDA is the wrong reference entirely during rapid weight loss, and what the actual target should be.

I would rather have one careful answer than five confident ones.

15 10mike_mealprep, NicoleRaleigh, james_edin and 12 others
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labquiet_amy
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Mar 10, 2026 at 7:22 AM#2

Taking the question as asked, rather than the general version of it. On a suppressed appetite the winning strategy is protein density per unit of volume, not per calorie. Isolate powders, Greek yoghurt and cottage cheese, egg whites, lean fish, and tinned tuna all deliver a lot of protein in a small physical volume. Front-load it: appetite is usually least suppressed in the first hours after waking and worst on the day or two after dosing, so get the majority in early in the day and early in the week.

I would rather be corrected than agreed with, if it comes to it.

Last edited: Mar 10, 2026 at 9:22 AM
14 9HPLC_Greg, LibrarianMeg, bri_stats and 11 others
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amsterdam_pete
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Mar 10, 2026 at 7:38 AM#3
labquiet_amy said:
On a suppressed appetite the winning strategy is protein density per unit of volume, not per calorie.

True, and anyone with reduced kidney function needs that target set by a clinician rather than a forum. High protein is safe in normal kidneys and is not automatically safe in impaired ones.

13 8CanadaChris, ZaraB_AL, JakeSmashed95 and 10 others
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ricardo_MIA
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Mar 10, 2026 at 7:54 AM#4
rick_sfbay said:
I tracked honestly for two weeks and averaged 68g of protein against a target of about 120g.

Can confirm the pattern rick_sfbay describes. The RDA is the wrong reference and it is worth understanding why. 0.8 g/kg is the intake at which nitrogen balance is not negative in healthy weight-stable adults — a floor for deficiency prevention, not an optimum, and derived in a population that is not in a deficit. In a substantial energy deficit, protein requirement rises because amino acids are being oxidised for energy and because muscle protein synthesis is blunted. The literature on preserving lean mass during weight loss lands around 1.4 to 2.0 g/kg of reference body weight, which is roughly two to two and a half times the RDA.

12 7KetoKyle, CanadaChris, ZaraB_AL and 9 others
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LipidDoc_ATL
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Mar 10, 2026 at 9:17 AM#5

Adding the clinical framing, because it changes how the question reads.

DEXA scan update relevant to protein intake:

Scanned at months 0, 6, and 12. Here are the body comp numbers:

BaselineMonth 6Month 12
Total mass248 lbs213 lbs205 lbs
Fat mass108 lbs68 lbs55 lbs
Lean mass161 lbs151 lbs152 lbs
Body fat %41%31%24%

Lean mass preserved — actually GAINED 1-2 lbs thanks to progressive overload + 1.5g/kg protein. The protein intake discussion needs to include body comp data, not just scale weight.

11 6KristenIndy, MarkLI_maint, Dr.PeteFamMed and 8 others
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