From the other side of the consultation, briefly. Order of operations matters more than any single choice here: establish a baseline, change one thing, wait long enough for it to express itself, then measure again under the same conditions.
hans_munich said:Order of operations matters more than any single choice here: establish a baseline, change one thing, wait long enough for it to express itself, then…
This matches mine closely enough to be worth saying so out loud. Nothing to add that would improve it.
hans_munich said:Order of operations matters more than any single choice here: establish a baseline, change one thing, wait long enough for it to express itself, then…
Adding the part of the answer the thread has not reached. The mechanism and the magnitude are separate questions. Agreeing that something happens says nothing about whether it happens enough to act on.
Certified reference materials, analytical reagents, and research-grade standards for peptide verification. Trusted by laboratories worldwide.
Shop Reference StandardsOne concrete data point for the thread. If you are comparing against somebody else’s result, check that you are comparing the same measurement taken the same way. Most of the apparent contradictions in these threads dissolve at that step.
OP back with an update, since a thread like this is useless without one.
Closing the loop. The answer turned out to depend on the detail I had left out of the opening post, which is a lesson about how to ask rather than about the subject.